Перегляд за автором "Kuchuk, N.V."

Результати 1-7 з 7

Comparison of different systems for purification of recombinant proteins produced by transient expression in plants

Lypova, N.M.; Sindarovska, Y.R.; Gerasymenko, I.M.; Sheludko, Y.V.; Bannikova, M.A.; Kuchuk, N.V. (Фактори експериментальної еволюції організмів, 2008)

Three different approaches for purification of transiently expressed recombinant proteins from plants using GFP as a reporter have been successfully applied. The purification scheme including ammonium sulfate precipitation ...
cyp11A1 canola plants under short time heat stress conditions

Sakhno, L.O.; Slyvets, M.S.; Kuchuk, N.V. (Цитология и генетика, 2014)

In order to investigate the high temperature tolerance of spring canola plants (Brassica napus L.) constitutively expressing cyp11A1 gene which encodes bovine cytochrome P450SCC the growth features were analyzed under short ...
Obtaining and analysis of товассо, lettuce and rape plants transformed with human interferon alfa 2b gene

Gerasymenko, I.M.; Lypova, N.M.; Sakhno, L.A.; Shcherbak, N.L.; Sindarovska, Y.R.; Bannikova, M.A.; Sheludko, Y.V.; Kuchuk, N.V. (Фактори експериментальної еволюції організмів, 2009)

Plasmid constructs for Agrobacterium-mediated transformation of plant nuclear genome with human interferon alpha2b gene were built up and used to obtain transgenic plants of tobacco, lettuce and rape. Presence of the ...
Obtaining of transgenic french bean plants (Phaseolus vulgaris L.) resistant to the herbicide pursuit by Agrobacterium-mediated transformation

Nifantova, S.N.; Komarnickiy, I.K.; Kuchuk, N.V. (Цитология и генетика, 2011)

The transgenic plants of French bean (Phaseolus vulgaris) resistant herbicide Pursuit and kanamycin have been obtained. The genetic transformation was carried out with Agrobacterium tumefaciens strain LBA4404 containing ...
Production of human interferon alfa 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression

Sindarovska, Y.R.; Gerasymenko, I.M.; Sheludko, Y.V.; Olevinskaya, Z.M.; Spivak, N.Y.; Kuchuk, N.V. (Цитология и генетика, 2010)

Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized ...
Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior

Sindarovska, Y.R.; Sheludko, Y.V.; Gerasymenko, I.M.; Bannikova, M.A.; Kuchuk, N.V. (2008)

Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium mediated transient expression in Nicotiana excelsior was applied for ...
Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins

Sindarovska, Y.R.; Gerasymenko, I.M.; Sheludko, Y.V.; Komarnytskyy, I.K.; Bannikova, M.A.; Kuchuk, N.V. (Цитология и генетика, 2005)

Hairy root cultures of Nicotiana benthamiana have been obtained by co-cultivation of leaf explants with Agrobacterium rhizogenes strain A4 harboring a binary vector plasmid, and transgenic nature of the obtained cultures ...

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Перегляд за автором "Kuchuk, N.V."

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